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Yes nearly all TNR programs spay pregnant cats and if this tom has only been around a while it is more likely that one or more of the females is in season.Hopefully the male will be able to be neutered soon as well,most programs don't give vaccinations that I have been with as well because you will never be able to revaccinate each year,which leads to my next answer,no most cats don't hate us for the operation they turn their hate towards the trap and sometimes the vet,they become trap wary,to get them in a trap again can be not impossible but hard.As for testing for the testing of diseases this is not done in my country but it is done in America in nearly all the programs that I have heard of,this is such a useful thing to do and as I said retrapping can be hard. Most cats go in the trap the first day or two,if not it is normally a 3 week battle ,persistance wins in the end

The wrong post count is normally a glitch in your account,go to your control centre,at the bottom of the page you will see where to go to report problems or see if other folk are having the same problems too,(ezmessage me if you are lost)

Good luck with the trapping I have all my fingers and toes crossed for a good result